Why does hormonal therapy affect miRNA levels?

Hormonal therapies (including estrogen-replacement therapy, testosterone supplementation, and hormonal contraceptives) alter the expression of numerous genes — including miRNA genes — across many tissues. Because the OncuraKit threshold is calibrated on a healthy population not on hormonal therapy, people currently on hormonal agents may have baseline miRNA levels that differ from the reference population, potentially affecting the accuracy of the 1.5× HL threshold for them specifically. This is why Yenos Analytical identifies people on hormonal therapy as a contraindication for the test. If you have recently completed hormonal therapy, speak with your physician about an appropriate waiting period before testing.