What is the advantage of amplification-free miRNA detection?

In PCR-based miRNA assays, a small amount of starting RNA is copied millions of times before measurement. Each copying cycle can introduce errors, and any variation in the efficiency of the amplification reaction gets exponentially magnified. The result is large variability in the final measurement — a phenomenon called 'PCR data spread.' This spread causes the measurement distributions for cancer and healthy samples to overlap, making it impossible to set a clean threshold. The Yenos nanopore platform measures each miRNA molecule directly without copying it — what you measure is exactly what is there in the sample. This eliminates amplification error entirely, producing the narrow, non-overlapping data distributions that enable the hard 1.5× HL threshold and zero data overlap.