What is the overall accuracy when all three markers are considered together?

The per-marker accuracy rates — miR-21 (93.8%), miR-375 (81.5%), miR-141 (97.3%) — represent how often each individual marker correctly identifies a cancer patient's sample as elevated. When three independent markers are used together, a cancer patient only needs at least one marker to be elevated for detection. The combined sensitivity is higher than any individual marker alone. Additionally, a second confirmatory run on the same sample for any elevated result pushes the specificity above 99.9% (fewer than 1 false positive in 1,000). The key distinguishing feature is zero data overlap: no healthy sample has scored above 1.5× HL in published Yenos studies, and no cancer sample has scored entirely below it across all three markers simultaneously.