What is the 'bracketing protocol' used by Yenos and why does it improve accuracy?

The Yenos bracketing protocol is a quality assurance method in which patient samples are interspersed with known calibration standards of defined miRNA concentration during a nanopore run. By alternating patient samples with calibration brackets (known concentrations of the target miRNA), the system can detect and correct for any drift in the 180 pA baseline current during a long experimental run, any variation in pore activity over time, and any batch-to-batch variation in osmium reagents. The calibration brackets provide 'a priori' accuracy — the measurement accuracy is verified against known standards within the same run rather than relying solely on historical reference values. This bracketing approach is one of Yenos's innovations for clinical-grade accuracy and is described in their published papers.