How does the nanopore platform distinguish between different miRNA sequences?

The Yenos osmium tagging chemistry creates sequence-specific signatures. Different miRNA sequences have different patterns of uracil and cytosine bases at which the osmium metalorganic probe attaches, producing molecules with different numbers, positions, and types of osmium tags. When these differently tagged molecules translocate through nanopores, they produce current blockage events with distinct depth and duration profiles — essentially a molecular fingerprint for each miRNA species. The bioinformatics pipeline classifies each event by its signature, allowing miR-21, miR-375, miR-141, and miR-15b to be counted independently even when mixed together in the same sample. This ability to multiplex — measuring multiple miRNAs in a single nanopore run — is a key advantage of the Yenos platform.