What nanopore protein is used in the MinION/Yenos system?

The standard MinION R9.4.1 flow cell uses the MspA-related or CsgG-based protein pore (Oxford Nanopore uses proprietary pore variants). These are naturally occurring bacterial channel proteins engineered for optimal electrical properties. The critical physical parameters for the Yenos application are: a pore diameter of approximately 1.4 nm (large enough for a single-stranded RNA to thread through but small enough to create measurable current blockage) and a stable, reproducible electrical behavior at the 180 pA baseline. Oxford Nanopore continues to develop new pore variants (R10.x), but Yenos's published validation was conducted using the R9.4.1 flow cell.